Zn2+ inhibits purified poly(ADP-ribose) polymerase (50% inhibition at 10 microM). Furthermore poly (ADP-ribose) polymerase present in nuclei and metaphase chromosome clusters is also inhibited by Zn2+. The inactivated enzyme could be re-activated by dithiothreitol. The concentration of Zn2+ needed to affect the enzyme activity in the organelles is sufficiently low for it to have a possible role in controlling the activity of this chromatin-bound enzyme.
The inhibitory effect of Zn2+ on poly(ADP-ribose) polymerase activity and its reversal
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A G Larsen, A C Ostvold, J Holtlund, T Kristensen, S G Laland; The inhibitory effect of Zn2+ on poly(ADP-ribose) polymerase activity and its reversal. Biochem J 1 May 1982; 203 (2): 511–513. doi: https://doi.org/10.1042/bj2030511
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