The quantitative significance of the conversion in vivo of L-[U-14C]leucine to ketone bodies was determined in rats starved for 3 or 48 h. In animals starved for 3 h, 4.4% of ketone-body carbon is derived from the metabolism of leucine, and in rats starved for 48 h the corresponding value is 2.3%. This conversion occurs rapidly, and the specific radioactivity of ketone bodies in blood is maximal at 2 min after the intravenous injection of labelled leucine for both periods of starvation. The flux of leucine in the blood is 1.01 and 1.04 mumol/min per 100 g body wt. respectively for animals starved for 3 and 48 h. The specific radioactivity of blood ketone bodies was compared at 2 min after the injection of labelled leucine, lysine and phenylalanine. The specific radioactivity was 4-5 fold higher with leucine than with lysine or phenylalanine.
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Research Article| May 15 1982
The role of leucine in ketogenesis in starved rats
Biochem J (1982) 204 (2): 399–403.
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L K Thomas, M Ittmann, C Cooper; The role of leucine in ketogenesis in starved rats. Biochem J 15 May 1982; 204 (2): 399–403. doi: https://doi.org/10.1042/bj2040399
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