Detergent-solubilized plasma-membrane proteins from lymphocytes of patients with chronic lymphocytic leukaemia were applied to phenylboronic acid-agarose. Some of the polypeptides were bound specifically and subsequently eluted with sorbitol. Immunoprecipitation analyses showed that no surface immunoglobulin M was bound, but that most of the histocompatibility antigens HLA-A, HLA-B, HLA-C and HLA-DR were.
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Research Article| July 01 1982
Fractionation of membrane proteins on phenylboronic acid-agarose
Biochem J (1982) 205 (1): 167–171.
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G T Williams, A P Johnstone, P D Dean; Fractionation of membrane proteins on phenylboronic acid-agarose. Biochem J 1 July 1982; 205 (1): 167–171. doi: https://doi.org/10.1042/bj2050167
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