The endogenous respiration of the rumen ciliate Dasytricha ruminantium maintained under an O2 tension of 2kPa (approximately 0.02 atm) was partially inhibited by KCN (40% inhibition) and NaN3 (58% inhibition). The organisms lack cytochromes, and sensitivity of respiration to KCN, NaN3, chloroquine and quercetin suggest that the operation of flavoprotein-iron-sulphur-mediated electron transport. As in Tritrichomonas foetus, hydrogenosomal respiration can be stimulated by the addition of CoA in the presence of 0.025% Triton X-100; stimulation by ADP was not detected. Stimulation of pyruvate-supported O2 uptake by Pi suggests that acetate is produced via acetyl phosphate.
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Research Article| August 15 1982
Respiration of the rumen ciliate Dasytricha ruminantium Schuberg
Biochem J (1982) 206 (2): 259–266.
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N Yarlett, D Lloyd, A G Williams; Respiration of the rumen ciliate Dasytricha ruminantium Schuberg. Biochem J 15 August 1982; 206 (2): 259–266. doi: https://doi.org/10.1042/bj2060259
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