Zinc release is the first quantitatively significant event detected during the triggering of Bacillus megaterium KM spore germination. Of the total spore Zn2+ pool 25% is released from non-heat-activated spores within 4 min of triggering germination. During this period only 10% of the spore population becomes irreversibly committed to germinate. The investigation of a putative role for Zn2+ in the germination trigger mechanism has established a relationship between the rate and extent of Zn2+ release and the stimulation of spore germination by heat activation. Furthermore, a correlation can be demonstrated between the extent of zinc release from spore populations and the time required to obtain 50% commitment of these populations to germinate over a wide temperature range. These findings have been used to expand a recently published model for the triggering of bacterial spore germination.
Zinc release and the sequence of biochemical events during triggering of Bacillus megaterium KM spore germination
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K Johnstone, G S A B Stewart, I R Scott, D J Ellar; Zinc release and the sequence of biochemical events during triggering of Bacillus megaterium KM spore germination. Biochem J 15 November 1982; 208 (2): 407–411. doi: https://doi.org/10.1042/bj2080407
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