The feasibility of using exogenous [3H]desmosterol as a mass metabolic tracer for exogenous non-esterified cholesterol in hepatocytes is investigated with albumin-bound non-esterified cholesterol containing [3H]desmosterol and [14C] cholesterol tracers. The amounts of uptake and metabolism of exogenous cholesterol monitored by either tracer are the same. In addition, the conversion of [3H]desmosterol into [3H]cholesterol by the delta 24-sterol reductase in the microsomes can be used as an estimate for the mass transfer of exogenous cholesterol to the microsomes. The results obtained indicate that only a small fraction of exogenous cholesterol that was transferred to the microsomes was metabolized into bile acids and steryl esters. The technique of estimating the mass transfer of exogenous cholesterol to the microsomes with [3H]desmosterol may be of importance in investigations dealing with the effect of exogenous plasma cholesterol on changes in the physiological functions of the endoplasmic reticulum in the cells.
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October 1983
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Research Article|
October 15 1983
Transfer of exogenous cholesterol to microsomes of hepatocytes investigated with [3H]desmosterol tracer
Biochem J (1983) 216 (1): 137–142.
Citation
E H Goh; Transfer of exogenous cholesterol to microsomes of hepatocytes investigated with [3H]desmosterol tracer. Biochem J 15 October 1983; 216 (1): 137–142. doi: https://doi.org/10.1042/bj2160137
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