Glyoxylate dehydrogenase (glyoxylate: NAD+ oxidoreductase) was purified 600-fold in three steps from crude extracts of the fungus Sclerotium rolfsii (Corticium rolfsii Curzi). Two of the purification steps involved dye-affinity chromatography. The enzyme is a tetramer of Mr 250 000, with identical subunits of Mr 57 000. Inhibition studies suggest that there is one essential thiol group per active site.
Research Article| February 15 1984
Isolation and characterization of glyoxylate dehydrogenase from the fungus Sclerotium rolfsii
Biochem J (1984) 218 (1): 113–118.
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A J Balmforth, A Thomson; Isolation and characterization of glyoxylate dehydrogenase from the fungus Sclerotium rolfsii. Biochem J 15 February 1984; 218 (1): 113–118. doi: https://doi.org/10.1042/bj2180113
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