Human thyroglobulin (Tg) could be adsorbed through one of its thyroxine (T4) residues by either of two T4-binding antibodies which had been covalently attached to Sepharose- CL4B . The antibodies used were (i) a purified human autoantibody specific for a T4-containing epitope in human Tg, or (ii) a rabbit antibody raised against T4 conjugated to bovine albumin side chains. Tg adsorbed by either immobilized antibody could then itself adsorb either type of antibody free in solution on to a further T4 residue. At least two T4 residues in human Tg are therefore sufficiently exposed to interact with T4-binding antibodies. Furthermore, these T4 residues are sufficiently far apart to allow the binding of two immunoglobulin molecules simultaneously. Previous observations of a marked preference by human autoantibodies for one of the T4-containing epitopes in Tg therefore reflect a higher binding energy with that epitope rather than an inability to interact with others. The T4-containing epitope which preferentially reacts with human Tg autoantibodies must therefore have a distinctive topography.
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Research Article| April 15 1984
Exposure of thyroxine residues in human thyroglobulin. Two-site binding studies
P G H Byfield ;
Biochem J (1984) 219 (2): 405–410.
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P G H Byfield, D Clingan, R L Himsworth; Exposure of thyroxine residues in human thyroglobulin. Two-site binding studies. Biochem J 15 April 1984; 219 (2): 405–410. doi: https://doi.org/10.1042/bj2190405
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