A theory is described for Rayleigh light-scattering from solutions of detergent-complexed macromolecules applicable to measurements carried out under conditions of Donnan equilibrium. The theory shows that when scattering measurements are made on detergent-solubilized macromolecules in the presence of detergent micelles the apparent Mr is dependent on the extent of detergent binding and effective charge on the detergent-macromolecule complex and the micellar charge and aggregation number. Equations are given for the apparent Mr of the macromolecule under limiting conditions of high salt and low salt concentration. Low-angle laser-light-scattering measurements were made on lysozyme complexed with sodium n-dodecyl sulphate both in the absence and in the presence of detergent micelles. These experimentally obtained data were used in conjunction with the detergent-binding isotherm to test the theory at high ionic strength. Light-scattering measurements were also made on detergent-saturated complexes as a function of ionic strength and pH. The results are in reasonable accord with both the qualitative and the quantitative predictions of the theory.
Research Article| May 01 1984
Light-scattering from detergent-complexed biological macromolecules
Biochem J (1984) 219 (3): 875–881.
- Views Icon Views
- Share Icon Share
M N Jones, P J W Midgley; Light-scattering from detergent-complexed biological macromolecules. Biochem J 1 May 1984; 219 (3): 875–881. doi: https://doi.org/10.1042/bj2190875
Download citation file: