The effects of verapamil, a calcium antagonist, on lipolysis in isolated rat adipocytes were studied. Verapamil (100 microM) potentiated lipolysis due to dibutyryl cyclic AMP (Bt2cAMP) at submaximal concentrations, with or without extracellular Ca2+. Lipolysis due to 0.5 mM-Bt2cAMP was potentiated by verapamil in a dose-dependent manner up to 200 microM, whereas at concentrations higher than 100 microM the stimulatory effect of verapamil was progressively diminished with or without extracellular Ca2+. Verapamil showed only an inhibitory effect on lipolysis due to adrenaline (0.1-10 microM) or 3-isobutyl-1-methylxanthine (IBMX; 25-200 microM). The stimulatory effect of verapamil on lipolysis due to Bt2cAMP was not blocked by alpha-adrenergic antagonists. These results suggest (i) that verapamil has a biphasic effect on lipolysis due to Bt2cAMP and only an inhibitory effect on that due to adrenaline or IBMX, and (ii) that extracellular Ca2+ or alpha-adrenergic receptors are not involved in the action of verapamil.
Skip Nav Destination
Research Article| May 15 1984
Effects of verapamil on lipolysis due to dibutyryl cyclic AMP
Biochem J (1984) 220 (1): 321–324.
- Views Icon Views
- Share Icon Share
H Goko, S Takashima, S Shimizu, S Kagawa, A Matsuoka; Effects of verapamil on lipolysis due to dibutyryl cyclic AMP. Biochem J 15 May 1984; 220 (1): 321–324. doi: https://doi.org/10.1042/bj2200321
Download citation file: