Hepatocyte cultures derived from 15-day foetal rats produce the liver form of pyruvate kinase (EC 184.108.40.206) only after 3 days of culture. The appearance of the liver form of the enzyme can be blocked by the addition of 5-bromodeoxyuridine on day 2 of culture, but not by addition on day 3 of culture. The reversibility of the action of 5-bromodeoxyuridine was shown when the inhibitor was added on day 2 and removed on day 4. By day 6 of culture the liver form of pyruvate kinase was detectable. The specificity of the action of 5-bromodeoxyuridine was monitored by following changes in the closely related embryonic form of the enzyme as a control. This was unaltered by the inhibitor.
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Research Article| June 15 1984
Effect of 5-bromodeoxyuridine on the appearance of the liver isoenzyme of pyruvate kinase
Biochem J (1984) 220 (3): 865–868.
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R Scott, G C T Yeoh; Effect of 5-bromodeoxyuridine on the appearance of the liver isoenzyme of pyruvate kinase. Biochem J 15 June 1984; 220 (3): 865–868. doi: https://doi.org/10.1042/bj2200865
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