Adenosine was converted into the arsonomethyl analogue of AMP. The reactions used provide a general route for converting an alcohol, R-CH2-OH, into the arsonomethyl analogue, R-CH2-CH2-AsO3H2, of its phosphate, R-CH2-O-PO3H2. The analogue of AMP proves to be a substrate for rabbit adenylate kinase, which shows a limiting velocity with it of 1/17 that with AMP, a Michaelis constant raised 70-fold to about 10 mM, and hence a specificity constant lowered about 1200-fold. The product of transfer of a phospho group from ATP to the analogue is, like all anhydrides of arsonic acids, unstable to hydrolysis, and so breaks down to yield orthophosphate and regenerate the analogue. Hence adenylate kinase is converted into an ATPase by the presence of the analogue.
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Research Article| August 01 1984
The arsonomethyl analogue of adenosine 5′-phosphate. An uncoupler of adenylate kinase
Biochem J (1984) 221 (3): 829–836.
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S R Adams, M J Sparkes, H B F Dixon; The arsonomethyl analogue of adenosine 5′-phosphate. An uncoupler of adenylate kinase. Biochem J 1 August 1984; 221 (3): 829–836. doi: https://doi.org/10.1042/bj2210829
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