In permeabilized hepatocytes, inositol 1,4,5-trisphosphate, inositol 2,4,5-trisphosphate and inositol 4,5-bisphosphate induced rapid release of Ca2+ from an ATP-dependent, non-mitochondrial vesicular pool, probably endoplasmic reticulum. The order of potency was inositol 1,4,5-trisphosphate greater than inositol 2,4,5-trisphosphate greater than inositol 4,5-bisphosphate. The Ca2+-releasing action of inositol 1,4,5-trisphosphate is not inhibited by high [Ca2+], nor is it dependent on [ATP] in the range of 50 microM-1.5 mM. These results suggest a role for inositol 1,4,5-trisphosphate as a second messenger in hormone-induced Ca2+ mobilisation, and that a specific receptor is involved in the Ca2+-release mechanism.
Research Article| December 15 1984
Actions of inositol phosphates on Ca2+ pools in guinea-pig hepatocytes
M J Berridge;
J S McKinney;
Biochem J (1984) 224 (3): 741–746.
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G M Burgess, R F Irvine, M J Berridge, J S McKinney, J W Putney; Actions of inositol phosphates on Ca2+ pools in guinea-pig hepatocytes. Biochem J 15 December 1984; 224 (3): 741–746. doi: https://doi.org/10.1042/bj2240741
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