The effect of acute inflammation on the production of the major phosphorylated protein (PP63) excreted by rat hepatocytes was investigated. Both intra- and extracellular forms of the protein labelled with [32P]Pi, [3H]fucose and [35S]methionine were immunoprecipitated with monospecific polyclonal antibodies, and relative rates of PP63 synthesis were measured. The hepatocytes of acutely inflamed rats produced and excreted 85% less 32P- and 3H-labelled PP63 than did control cells. This decreased amount of PP63 did not result from an impairment in the phosphorylation or glycosylation processes or from a blockade in excretion, but rather was found to be due to extensive shut-off in biosynthesis of the protein as measured by [35S]methionine incorporation. Thus PP63 would appear to represent a new negatively regulated acute-phase protein.
Research Article| September 15 1985
A new negatively regulated acute-phase phosphoprotein synthesized by rat hepatocytes
Biochem J (1985) 230 (3): 603–607.
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A Le Cam, G Le Cam; A new negatively regulated acute-phase phosphoprotein synthesized by rat hepatocytes. Biochem J 15 September 1985; 230 (3): 603–607. doi: https://doi.org/10.1042/bj2300603
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