We report for the first time a masticatory-lubrication assay system to assess the lubricating properties of salivary constituents. The lubricating ability of the proline-rich glycoprotein (PRG) of parotid saliva was enhanced by human serum albumin. The interactive effect of albumin was abolished by chemically deglycosylating the glycoprotein. Fluorescence spectroscopy with a hydrophobic probe verified the existence of a PRG-albumin complex and demonstrated that deglycosylation of the PRG altered the nature of its interaction with albumin.
Masticatory lubrication. The role of carbohydrate in the lubricating property of a salivary glycoprotein-albumin complex
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M N Hatton, R E Loomis, M J Levine, L A Tabak; Masticatory lubrication. The role of carbohydrate in the lubricating property of a salivary glycoprotein-albumin complex. Biochem J 15 September 1985; 230 (3): 817–820. doi: https://doi.org/10.1042/bj2300817
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