[2-18O]Ribulose 5-phosphate was prepared and shown to be converted enzymically by 6-phosphogluconate dehydrogenase from sheep liver into 6-phosphogluconate with complete retention of the heavy isotope. This finding unequivocally excludes the possibility of a Schiff-base mechanism for the enzyme. The involvement of metal ions has already been excluded, and other possible mechanisms are discussed. The enzyme was purified by an improved large-scale procedure, which is briefly described.
Skip Nav Destination
Research Article| March 15 1986
The chemical mechanism of sheep liver 6-phosphogluconate dehydrogenase. A Schiff-base intermediate is not involved
Biochem J (1986) 234 (3): 671–677.
- Views Icon Views
- Share Icon Share
C M Topham, K Dalziel; The chemical mechanism of sheep liver 6-phosphogluconate dehydrogenase. A Schiff-base intermediate is not involved. Biochem J 15 March 1986; 234 (3): 671–677. doi: https://doi.org/10.1042/bj2340671
Download citation file: