The mechanism of the enzymic isomerization and epimerization of D-erythrose 4-phosphate (Ery4P) by an enzyme preparation from bovine liver was investigated with the use of 2H2O. The incorporation of 2H was quantitatively determined by a procedure using gas chromatography-mass spectrometry. About one atom of 2H was incorporated per molecule of the enzymic epimerization reaction product of Ery4P (D-threose 4-phosphate) or that of D-ribulose 5-phosphate. Computer simulation of the Ery4P isomerization reaction indicated that the 2H of 2H2O was not directly incorporated into the enzymic reaction product (D-erythrulose 4-phosphate). Instead, intramolecular transfer of hydrogen atoms had occurred.
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Research Article| November 01 1986
Mechanism of enzymic isomerization and epimerization of d-erythrose 4-phosphate
Biochem J (1986) 239 (3): 739–743.
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S Hosomi, N Nakai, J Kogita, T Terada, T Mizoguchi; Mechanism of enzymic isomerization and epimerization of d-erythrose 4-phosphate. Biochem J 1 November 1986; 239 (3): 739–743. doi: https://doi.org/10.1042/bj2390739
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