A cDNA clone (HLUG 25) encoding the complete sequence of a human liver UDP-glucuronosyltransferase was isolated from a lambda gt11 human liver cDNA library. The library was screened by hybridization to a partial-length human UDP-glucuronosyltransferase cDNA (pHUDPGT1) identified from a human liver pEX cDNA expression library by using anti-UDP-glucuronosyltransferase antibodies. The authenticity of the cDNA clone was confirmed by hybrid-select translation and extensive sequence homology to rat liver UDP-glucuronosyltransferase cDNAs. The sequence of HLUG 25 cDNA was determined to be 2104 base-pairs long, including a poly(A) tail, and contains a long open reading frame. The possible site of translation initiation of this sequence is discussed with reference to a rat UDP-glucuronosyltransferase cDNA clone (RLUG 38).
Cloning of a human liver microsomal UDP-glucuronosyltransferase cDNA
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M R Jackson, L R McCarthy, D Harding, S Wilson, M W H Coughtrie, B Burchell; Cloning of a human liver microsomal UDP-glucuronosyltransferase cDNA. Biochem J 1 March 1987; 242 (2): 581–588. doi: https://doi.org/10.1042/bj2420581
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