beta-Lactamase K1 was purified from Klebsiella pneumoniae SC10436. It is very similar to the enzyme produced by Klebsiella aerogenes 1082E and described by Emanuel, Gagnon & Waley [Biochem. J. (1986) 234, 343-347]. An active-site peptide was isolated after labelling of the enzyme with tritiated beta-iodopenicillanate. A cysteine residue was found just before the active-site serine residue. This result could explain the properties of the enzyme after modification by thiol-blocking reagents. The sequence of the active-site peptide clearly established the enzyme as a class A beta-lactamase.
Research Article| April 15 1987
The K1 β-lactamase of Klebsiella pneumoniae
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Biochem J (1987) 243 (2): 561–567.
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B Joris, F De Meester, M Galleni, J M Frère, J Van Beeumen; The K1 β-lactamase of Klebsiella pneumoniae. Biochem J 15 April 1987; 243 (2): 561–567. doi: https://doi.org/10.1042/bj2430561
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