The active-site serine of the extracellular beta-lactamases of Streptomyces cacaoi and Streptomyces albus G has been labelled with beta-iodopenicillanate. The determination of the sequence of the labelled peptides obtained after trypsin digestion of the denatured proteins indicate both enzymes to be class A beta-lactamases. Surprisingly the two Streptomyces enzymes do not appear to be especially homologous, and none of them exhibited a high degree of homology with the Streptomyces R61 DD-peptidase. Our data confirm that, as a family of homologous enzymes, class A is rather heterogeneous, with only a small number of conserved residues in all members of the class.
The active sites of the β-lactamases of Streptomyces cacaoi and Streptomyces albus G.
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F De Meester, B Joris, M V Lenzini, P Dehottay, T Erpicium, J Dusart, D Klein, J M Ghuysen, J M Frère, J Van Beeumen; The active sites of the β-lactamases of Streptomyces cacaoi and Streptomyces albus G.. Biochem J 1 June 1987; 244 (2): 427–432. doi: https://doi.org/10.1042/bj2440427
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