The continuous alpha-neurotoxin-binding regions on the extracellular part (residues 1-210) of the alpha-chain of Torpedo californica acetylcholine receptor were localized by reaction of 125I-labelled alpha-bungarotoxin with synthetic overlapping peptides spanning this entire part of the chain. The specificity of the binding was confirmed by inhibition with unlabelled toxin and, for appropriate peptides, with unlabelled anti-(acetylcholine receptor) antibodies. Five toxin-binding regions were localized within residues 1-10, 32-41, 100-115, 122-150 and 182-198. The third, fourth and fifth (and to a lesser extent the first and second) toxin-binding regions overlapped with regions recognized by anti-(acetylcholine receptor) antibodies. The five toxin-binding regions may be distinct sites or, alternatively, different ‘faces’ in one (or more) sites.
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December 1987
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Research Article|
December 15 1987
Profile of the α-bungarotoxin-binding regions on the extracellular part of the α-chain of Torpedo californica acetylcholine receptor
B MULAC-JERIČEVIČ;
B MULAC-JERIČEVIČ
Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030, U.S.A.
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M Z Atassi
M Z Atassi
Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030, U.S.A.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1987 London: The Biochemical Society
1987
Biochem J (1987) 248 (3): 847–852.
Citation
B MULAC-JERIČEVIČ, M Z Atassi; Profile of the α-bungarotoxin-binding regions on the extracellular part of the α-chain of Torpedo californica acetylcholine receptor. Biochem J 15 December 1987; 248 (3): 847–852. doi: https://doi.org/10.1042/bj2480847
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