We describe the isolation by reversed-phase h.p.l.c. of a number of products of the degradation of insulin by insulin proteinase and their direct analysis by fast atom bombardment mass spectrometry (f.a.b.-m.s.). Various semisynthetically labelled insulins were used, including [[2H2]GlyA1]insulin and [18O]LysB29]insulin. The results obtained confirm and extend the results obtained by non-mass-spectrometric methods [Davies, Muir, Rose & Offord (1988) Biochem. J. 249, 209-214, and papers cited therein]. Cleavage sites were identified between positions A13-A14, A14-A15, B9-B10, B13-B14, B24-B25 and B25-B26. The advantages and disadvantages of the application of f.a.b.-m.s. to such studies are discussed.
Identification by fast atom bombardment mass spectrometry of insulin fragments produced by insulin proteinase
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L A Savoy, R M L Jones, S Pochon, J G Davies, A V Muir, R E Offord, K Rose; Identification by fast atom bombardment mass spectrometry of insulin fragments produced by insulin proteinase. Biochem J 1 January 1988; 249 (1): 215–222. doi: https://doi.org/10.1042/bj2490215
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