Biotinidase shows two binding sites for biotin, with Kd = 59 and 3 nM respectively, and requires tryptophan and cysteine residues of the biotinidase protein for biotin-binding activity. Analysis of human serum by various column-chromatographic techniques indicates that biotinidase is the only protein which exchanges with labelled (+)-biotin. It was shown previously that epileptic patients receiving a high average dose of anticonvulsants (containing a carbamide group) have lower biotin concentrations than those receiving a low dose. We have shown in human serum and with purified biotinidase that these anticonvulsant drugs compete with biotin for binding to the protein moiety.
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Research Article| November 15 1988
Role of human serum biotinidase as biotin-binding protein
Biochem J (1988) 256 (1): 265–270.
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J Chauhan, K Dakshinamurti; Role of human serum biotinidase as biotin-binding protein. Biochem J 15 November 1988; 256 (1): 265–270. doi: https://doi.org/10.1042/bj2560265
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