Smooth microsomal membranes, prepared from rat liver by sucrose-density-gradient centrifugation, were subfractionated by counter-current distribution in an aqueous two-phase system consisting of poly(ethylene glycol) and Dextran T500. A comparison of the distribution curves of marker enzymes, together with theoretically calculated curves, indicated the presence of at least five membrane subfractions, differing in the ratios of the marker enzymes. Glucose-6-phosphatase and arylesterase distributed in one manner, and NADPH-cytochrome c reductase and NADH-ferricyanide reductase in another. Evidence for further heterogeneities in the distribution of marker enzymes in smooth microsomes was obtained by analysing the membrane domain structure using a recently described method [Albertsson (1988) Q. Rev. Biophys. 21, 61-98]. Phenobarbital treatment did not influence the behaviour of the marker enzymes.
Skip Nav Destination
Article navigation
August 1989
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Research Article|
August 15 1989
Heterogeneity of smooth endoplasmic reticulum from rat liver studied by two-phase partitioning
P Gierow;
P Gierow
1Department of Biochemistry, Chemical Centre, University of Lund, P.O. Box 124, S-221 00 Lund, Sweden.
Search for other works by this author on:
B Jergil
B Jergil
1Department of Biochemistry, Chemical Centre, University of Lund, P.O. Box 124, S-221 00 Lund, Sweden.
Search for other works by this author on:
Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1989 London: The Biochemical Society
1989
Biochem J (1989) 262 (1): 55–61.
Citation
P Gierow, B Jergil; Heterogeneity of smooth endoplasmic reticulum from rat liver studied by two-phase partitioning. Biochem J 15 August 1989; 262 (1): 55–61. doi: https://doi.org/10.1042/bj2620055
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.