Escherichia coli [formamidopyrimidine]DNA glycosylase catalyses the nicking of both the phosphodiester bonds 3′ and 5′ of apurinic or apyrimidinic sites in DNA so that the base-free deoxyribose is replaced by a gap limited by 3′-phosphate and 5′-phosphate ends. The two nickings are not the results of hydrolytic processes; the [formamidopyrimidine]DNA glycosylase rather catalyses a beta-elimination reaction that is immediately followed by a delta-elimination. The enzyme is without action on a 3′-terminal base-free deoxyribose or on a 3′-terminal base-free unsaturated sugar produced by a beta-elimination reaction nicking the DNA strand 3′ to an apurinic or apyrimidinic site.
Mechanism of DNA strand nicking at apurinic/apyrimidinic sites by Escherichia coli [formamidopyrimidine]DNA glycosylase
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V Bailly, W G Verly, T O'Connor, J Laval; Mechanism of DNA strand nicking at apurinic/apyrimidinic sites by Escherichia coli [formamidopyrimidine]DNA glycosylase. Biochem J 1 September 1989; 262 (2): 581–589. doi: https://doi.org/10.1042/bj2620581
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