Three major autophosphorylation sites are located near the C-terminus of the epidermal growth factor receptor, but a fourth site is repeatedly detected. We report here the purification and sequencing of a tryptic peptide containing this site, Tyr-1086. Furthermore, we demonstrate that additional phosphopeptides are observed following both partial digestion and overdigestion. Finally, we show that Tyr-1086 can be phosphorylated in intact cells.
Research Article| September 01 1989
Identification of a novel autophosphorylation site (P4) on the epidermal growth factor receptor
J J Hsuan;
Biochem J (1989) 262 (2): 659-663.
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J J Hsuan, N Totty, M D Waterfield; Identification of a novel autophosphorylation site (P4) on the epidermal growth factor receptor. Biochem J 1 September 1989; 262 (2): 659–663. doi: https://doi.org/10.1042/bj2620659
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