The phospholipase C-catalysed breakdown of inositol-containing phospholipids is an important source of diacylglycerol in cells stimulated by several agonists. However, recent experimental evidence suggests that major phospholipids such as phosphatidylcholine may also be substrates of the phosphodiesteratic hydrolysis activated by hormones, growth factors and oncogene products. We show here that stimulation of muscarinic agonists activates the release of phosphocholine, which, along with diacylglycerol, is a metabolic product of phospholipase C-mediated hydrolysis of phosphatidylcholine. Fluoroaluminates mimic this muscarinic effect, strongly suggesting that carbachol-activated release of phosphocholine may be mediated by a guanine-nucleotide-binding protein. Evidence for this was obtained from experiments using permeabilized cells in which non-hydrolysable analogues of GTP activated phosphocholine release synergistically with carbachol.
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October 1989
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Research Article|
October 01 1989
Phospholipase C-mediated hydrolysis of phosphatidylcholine is activated by muscarinic agonists
M T Diaz-Meco;
M T Diaz-Meco
1Medicina y Cirugia Experimental, Hospital General ‘Gregorio Maranon’, Dr. Esquerdo 46, 28007 Madrid, Spain.
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P Larrodera;
P Larrodera
1Medicina y Cirugia Experimental, Hospital General ‘Gregorio Maranon’, Dr. Esquerdo 46, 28007 Madrid, Spain.
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M Lopez-Barahona;
M Lopez-Barahona
1Medicina y Cirugia Experimental, Hospital General ‘Gregorio Maranon’, Dr. Esquerdo 46, 28007 Madrid, Spain.
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M E Cornet;
M E Cornet
1Medicina y Cirugia Experimental, Hospital General ‘Gregorio Maranon’, Dr. Esquerdo 46, 28007 Madrid, Spain.
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P G Barreno;
P G Barreno
1Medicina y Cirugia Experimental, Hospital General ‘Gregorio Maranon’, Dr. Esquerdo 46, 28007 Madrid, Spain.
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J Moscat
J Moscat
1Medicina y Cirugia Experimental, Hospital General ‘Gregorio Maranon’, Dr. Esquerdo 46, 28007 Madrid, Spain.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1989 London: The Biochemical Society
1989
Biochem J (1989) 263 (1): 115–120.
Citation
M T Diaz-Meco, P Larrodera, M Lopez-Barahona, M E Cornet, P G Barreno, J Moscat; Phospholipase C-mediated hydrolysis of phosphatidylcholine is activated by muscarinic agonists. Biochem J 1 October 1989; 263 (1): 115–120. doi: https://doi.org/10.1042/bj2630115
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