Newborn Wistar rats were made hyperthyroid by injection of tri-iodothyronine and assayed for survival, brain oxygen uptake, brain chemiluminescence and activity of antioxidant enzymes. Brain chemiluminescence was measured (1) by removing the parietal bones or (2) through the translucid parietal bones. Control animals showed a brain chemiluminescence of 130 +/- 12 c.p.s./cm2 and 99 +/- 10 c.p.s./cm2 for procedures (1) and (2) respectively. Hyperthyroid rats showed increases in the spontaneous brain photoemission of 46 and 70% compared with controls, measured by procedures 1 and 2 respectively. The hyperthyroid state did not modify the oxygen-dependent chemiluminescence of brain homogenates. The hyperthyroid animals showed a 30% increase in the oxygen uptake of brain slices and a dramatic shortening of life-span to about 16 weeks. Superoxide dismutase (the Cu-Zn enzyme), catalase and Se-dependent glutathione peroxidase activities of brain homogenates were increased by 18, 36 and 30% respectively in the hyperthyroid animals. Isolated brain mitochondria produced 0.18-0.20 nmol of H2O2/min per mg of protein in state 4 in the presence of succinate as substrate. No difference was observed between control and hyperthyroid animals. It is concluded that hyperthyroidism leads to hypermetabolism and oxidative stress in the brain. The increased levels of oxygen and peroxyl radicals may contribute to premature ageing in these animals.
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October 1989
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Research Article|
October 01 1989
Brain chemiluminescence and oxidative stress in hyperthyroid rats
A M Adamo;
A M Adamo
1Departamento de Quimica Biologica-IQUIFIB, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires-CONICET, Junin 956, 1113 Buenos Aires, Argentina.
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S F Llesuy;
S F Llesuy
1Departamento de Quimica Biologica-IQUIFIB, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires-CONICET, Junin 956, 1113 Buenos Aires, Argentina.
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J M Pasquini;
J M Pasquini
1Departamento de Quimica Biologica-IQUIFIB, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires-CONICET, Junin 956, 1113 Buenos Aires, Argentina.
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A Boveris
A Boveris
1Departamento de Quimica Biologica-IQUIFIB, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires-CONICET, Junin 956, 1113 Buenos Aires, Argentina.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1989 London: The Biochemical Society
1989
Biochem J (1989) 263 (1): 273–277.
Citation
A M Adamo, S F Llesuy, J M Pasquini, A Boveris; Brain chemiluminescence and oxidative stress in hyperthyroid rats. Biochem J 1 October 1989; 263 (1): 273–277. doi: https://doi.org/10.1042/bj2630273
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