Neutrophils stimulated with formylmethionyl-leucylphenylalanine (fMet-Leu-Phe) in the presence of butanol and ethanol formed phosphatidyl alcohols through a phospholipase D mechanism. The alcohols inhibited phosphatidic acid and diradylglycerol (DRG) formation, but did not block inositol 1, 4, 5-trisphosphate release. fMet-Leu-Phe-stimulated superoxide production was inhibited by alcohol concentrations which blocked DRG formation, whereas opsonized-zymosan-stimulated superoxide production was only partially decreased. These results suggest that phospholipase D activation is functionally linked to superoxide production in the human neutrophil.
Phospholipase D activation is functionally linked to superoxide generation in the human neutrophil
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R W Bonser, N T Thompson, R W Randall, L G Garland; Phospholipase D activation is functionally linked to superoxide generation in the human neutrophil. Biochem J 1 December 1989; 264 (2): 617–620. doi: https://doi.org/10.1042/bj2640617
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