Exposure of isolated single-pass-perfused rat liver to hypo-osmotic media resulted in liver cell swelling and an inhibition of release of branched-chain amino acids. Similarly, cell swelling inhibited [3H]leucine release from perfused livers from rats in which liver proteins were prelabelled in vivo by intraperitoneal injection of L-[4,5-3H]leucine 16-20 h before the experiment. The effects of cell swelling on [3H]leucine release were fully reversible. [3H]Leucine release was also inhibited when cell swelling was induced by addition of glutamine (0.5-2 mM). There was a close relationship between the inhibition of [3H]leucine release and the degree of liver cell swelling, regardless of whether cell swelling was induced by hypo-osmotic perfusion or addition of glutamine. The data suggest that the known anti-proteolytic effect of glutamine is in large part due to glutamine-induced hepatocyte swelling.
Research Article| November 15 1990
Cell swelling inhibits proteolysis in perfused rat liver
S vom Dahl;
Biochem J (1990) 272 (1): 239-242.
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D Häussinger, C Hallbrucker, S vom Dahl, F Lang, W Gerok; Cell swelling inhibits proteolysis in perfused rat liver. Biochem J 15 November 1990; 272 (1): 239–242. doi: https://doi.org/10.1042/bj2720239
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