We demonstrate that annexin I/lipocortin I (lipo I) gene expression is regulated by dexamethasone (DEX) in mouse 3T3-L1 fibroblasts and LA-4 lung epithelial cells. We have characterized this induction further in 3T3-L1 fibroblasts. At 24 h after addition of DEX, the levels of lipo I mRNA and protein increased 5-fold and 1.5-fold respectively. Time-course experiments revealed that the induction was delayed by 2-4 h after DEX addition. Half-maximal induction of both lipo I mRNA and protein was achieved with 10 nM-DEX. Both actinomycin D and cycloheximide blocked the DEX effect on lipo I mRNA expression. These results indicate that the induction of lipo I by DEX has a transcriptional component and requires protein synthesis de novo.
Research Article| April 15 1991
Protein-synthesis-dependent induction of annexin I by glucocorticoid
W T Wong;
S C Frost;
Biochem J (1991) 275 (2): 313–319.
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W T Wong, S C Frost, H S Nick; Protein-synthesis-dependent induction of annexin I by glucocorticoid. Biochem J 15 April 1991; 275 (2): 313–319. doi: https://doi.org/10.1042/bj2750313
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