A recombinant pig ribonuclease inhibitor (delta r-RI) lacking 90 or 93 N-terminal amino acid residues was isolated from a preparation of recombinant inhibitor. The kinetic parameters for the inhibition of ribonuclease A by delta r-RI were determined and found to be only slightly altered in comparison with the full-length inhibitor. The deletion did, however, affect the surface properties of RI. The results are discussed in relation to those obtained by Lee & Vallee [(1990) Proc. Natl. Acad. Sci. U.S.A. 87, 1879-1883].
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Research Article| April 15 1991
Purification and characterization of truncated ribonuclease inhibitor
J Hofsteenge ;
A Vincentini ;
Biochem J (1991) 275 (2): 541–543.
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J Hofsteenge, A Vincentini, S R Stone; Purification and characterization of truncated ribonuclease inhibitor. Biochem J 15 April 1991; 275 (2): 541–543. doi: https://doi.org/10.1042/bj2750541
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