Gluconeogenesis was studied in hepatocytes isolated from phenobarbital-pretreated rats fasted for 24 h. In closed vial incubations, glucose production from lactate (20 mmol/l) and pyruvate (2 mmol/l), alanine (20 mmol/l) or glutamine (20 mmol/l) was suppressed by about 30-45%, although glycerol metabolism was not affected. In hepatocytes perifused with lactate and pyruvate (ratio 10:1), glucose production was inhibited by 50%, even at low gluconeogenic flux. From the determination of gluconeogenic intermediates at several steady states of gluconeogenic flux, we have found a single relationship between phosphoenolpyruvate and the rate of glucose production (Jglucose), and two different curves between cytosolic oxaloacetate and Jglucose in controls and in phenobarbital-pretreated hepatocytes. By using 3-mercaptopicolinate to determine the flux control coefficient of phosphoenolpyruvate carboxykinase we found that phenobarbital pretreatment led to an increase in this coefficient from 0.3 (controls) to 0.8 (phenobarbital group). These observations were confirmed by the finding that the activity of phosphoenolpyruvate carboxykinase was decreased by 50% after phenobarbital treatment. Hence we conclude that the inhibitory effect of phenobarbital on gluconeogenesis is due, at least partly, to a decrease in the flux through phosphoenolpyruvate carboxykinase.
Skip Nav Destination
Follow us on Twitter @Biochem_Journal
Article navigation
December 1991
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Research Article|
December 15 1991
Inhibition of gluconeogenesis in isolated rat hepatocytes after chronic treatment with phenobarbital
D Argaud;
D Argaud
*Laboratoire de Thérapeutique, Université Joseph Fourier, Bât. 72 Biologie, BP 53X, 38041 Grenoble-Cedex
Search for other works by this author on:
S Halimi;
S Halimi
†Service de Diabétologie, Hôpital A. Michallon, BP 217, 38043 Grenoble-Cedex 9, France
Search for other works by this author on:
F Catelloni;
F Catelloni
*Laboratoire de Thérapeutique, Université Joseph Fourier, Bât. 72 Biologie, BP 53X, 38041 Grenoble-Cedex
Search for other works by this author on:
X M Leverve
X M Leverve
*Laboratoire de Thérapeutique, Université Joseph Fourier, Bât. 72 Biologie, BP 53X, 38041 Grenoble-Cedex
Search for other works by this author on:
Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1991 The Biochemical Society, London
1991
Biochem J (1991) 280 (3): 663–669.
Citation
D Argaud, S Halimi, F Catelloni, X M Leverve; Inhibition of gluconeogenesis in isolated rat hepatocytes after chronic treatment with phenobarbital. Biochem J 15 December 1991; 280 (3): 663–669. doi: https://doi.org/10.1042/bj2800663
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Follow us on Twitter @Biochem_Journal
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() View past webinars > |