A novel location of the bile-acid-conjugating enzyme bile acid-CoA:amino acid N-acyltransferase (BAT) has been discovered in the cytosolic fraction of rat kidney. Both taurine and glycine were utilized as substrates. Formation of bile acid N-acyl amidates was verified by h.p.l.c. by comparison with authentic standards and by specific hydrolysis using cholylglycine hydrolase. Immunoblot analysis using a human liver anti-BAT polyclonal antibody indicated that rat kidney BAT has the same molecular mass as rat liver BAT. These findings suggest that the kidney has a role in bile acid metabolism and physiology.
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Research Article| December 15 1991
Identification of bile acid-CoA: amino acid N-acyltransferase in rat kidney
J B Kwakye ;
M R Johnson ;
S Barnes ;
W E Grizzle ;
Biochem J (1991) 280 (3): 821–824.
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J B Kwakye, M R Johnson, S Barnes, W E Grizzle, R B Diasio; Identification of bile acid-CoA: amino acid N-acyltransferase in rat kidney. Biochem J 15 December 1991; 280 (3): 821–824. doi: https://doi.org/10.1042/bj2800821
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