Incubation of rat hepatocytes with glucose results in a decrease in the amount of glycogen synthase activity found in supernatants obtained after centrifugation of cell homogenates at 9200 g. The enzymic activity was quantitatively recovered in the sediments. This effect of translocation was dose- and time-dependent and correlated with the amount of immunoreactive enzyme determined by immunoblotting in both fractions. Hydrolysis by alpha-amylase of glycogen accumulated upon incubation with the sugar did not affect the translocation pattern. Translocation was also observed when cells were incubated with 2-deoxyglucose, which did not result in accumulation of glycogen. Immunocytochemical evidence indicates that glucose induces the aggregation of glycogen synthase molecules into clusters which are recovered in the sediments. These results indicate that glucose, in addition to activating glycogen synthase, may trigger changes in the localization of the enzyme in the cell.
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January 1992
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Research Article|
January 15 1992
Glucose induces the translocation and the aggregation of glycogen synthase in rat hepatocytes
J M Fernández-Novell;
J M Fernández-Novell
*Departament de Bioquímica i Fisiologia, Universitat de Barcelona, E-08028 Barcelona
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J Ariño;
J Ariño
†Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain
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S Vilaró;
S Vilaró
*Departament de Bioquímica i Fisiologia, Universitat de Barcelona, E-08028 Barcelona
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J J Guinovart
J J Guinovart
*Departament de Bioquímica i Fisiologia, Universitat de Barcelona, E-08028 Barcelona
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1992 The Biochemical Society, London
1992
Biochem J (1992) 281 (2): 443–448.
Citation
J M Fernández-Novell, J Ariño, S Vilaró, J J Guinovart; Glucose induces the translocation and the aggregation of glycogen synthase in rat hepatocytes. Biochem J 15 January 1992; 281 (2): 443–448. doi: https://doi.org/10.1042/bj2810443
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