Vascular endothelial cells, smooth muscle cells, macrophages, neutrophils, Kupffer cells and other diverse cell types generate superoxide (O2.-) and nitric oxide (.NO), which can react to form the potent oxidant peroxynitrite anion (ONOO-). Peroxynitrite reacted with luminol to yield chemiluminescence which was greatly enhanced by bicarbonate. The quantum chemiluminescence yield of the ONOO- reaction with luminol in bicarbonate was approx. 10(-3). Chemiluminescence was superoxide dismutase-inhibitable, indicating that O2.- was a key intermediate for chemiexcitation. O2.- appears to be formed secondarily to the reaction of a bicarbonate-peroxynitrite complex with luminol, yielding luminol radical and O2.-. Luminol radical reacts with O2.- to form the unstable luminol endoperoxide, which follows the light-emitting pathway. Neither .NO nor O2.- alone were capable of directly inducing significant luminol chemiluminescence in our assay systems. These results suggest that ONOO- can be a critical unrecognized mediator of cell-derived luminol chemiluminescence reported in previous studies. In addition, it is shown that bicarbonate can participate in secondary oxidation reactions after reacting with ONOO-.
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February 1993
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Research Article|
February 15 1993
Peroxynitrite-induced luminol chemiluminescence Available to Purchase
R Radi;
R Radi
*Department of Anesthesiology, University of Alabama at Birmingham, Birmingham, AL 35233-6810, U.S.A.
†Biochemistry, University of Alabama at Birmingham, Birmingham, AL 35233-6810, U.S.A.
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T P Cosgrove;
T P Cosgrove
*Department of Anesthesiology, University of Alabama at Birmingham, Birmingham, AL 35233-6810, U.S.A.
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J S Beckman;
J S Beckman
*Department of Anesthesiology, University of Alabama at Birmingham, Birmingham, AL 35233-6810, U.S.A.
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B A Freeman
B A Freeman
*Department of Anesthesiology, University of Alabama at Birmingham, Birmingham, AL 35233-6810, U.S.A.
†Biochemistry, University of Alabama at Birmingham, Birmingham, AL 35233-6810, U.S.A.
§Pediatrics, University of Alabama at Birmingham, Birmingham, AL 35233-6810, U.S.A.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1993 The Biochemical Society, London
1993
Biochem J (1993) 290 (1): 51–57.
Citation
R Radi, T P Cosgrove, J S Beckman, B A Freeman; Peroxynitrite-induced luminol chemiluminescence. Biochem J 15 February 1993; 290 (1): 51–57. doi: https://doi.org/10.1042/bj2900051
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