A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.
A new, highly sensitive method for the detection and quantification of penicillin-binding proteins
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M Galleni, B Lakaye, S Lepage, M Jamin, I Thamm, B Joris, J M Frère; A new, highly sensitive method for the detection and quantification of penicillin-binding proteins. Biochem J 1 April 1993; 291 (1): 19–21. doi: https://doi.org/10.1042/bj2910019
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