Metabolite channelling may result from different kinetic mechanisms in which enzyme-enzyme interactions occur, so that intermediates are not released into the bulk solution and cannot be used by enzymes outside the channel. From an evolutionary point of view, the emergence of such mechanisms may provide new functional possibilities for the system, which would result in a selective advantage. Hence, it would be useful to evaluate the objective advantages provided by the various options by considering different criteria for functional effectiveness. Following this strategy, the goal of this paper is to compare a model for a free-diffusion two-enzyme system with two different models with inclusion of enzyme-enzyme interactions. In addition, models with simultaneous free and interacting branches are also analysed, and their advantages or disadvantages are presented. Basic guidelines are suggested that help in predicting the occurrence of specific mechanisms in different circumstances, and provide theoretical evidence in support of the hypothesis that no single solution simultaneously optimizes all the possible desired properties of the system.

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