The regulatory properties of NAD(+)-isocitrate dehydrogenase and oxoglutarate dehydrogenase in extracts of yeast and rat heart mitochondria were studied under identical conditions. Yeast NAD(+)-isocitrate dehydrogenase exhibits a low K0.5 for isocitrate and is activated by AMP and ADP, but is insensitive to ATP and Ca2+. In contrast, the rat heart NAD(+)-isocitrate dehydrogenase was insensitive to AMP, but was activated by ADP and by Ca2+ in the presence of ADP or ATP. Both yeast and rat heart oxoglutarate dehydrogenase were stimulated by ADP, but only the heart enzyme was activated by Ca2+. All the enzymes studied were activated by decreases in pH, but to differing extents. The effects of Ca2+, adenine nucleotides and pH were through K0.5 for isocitrate or 2-oxoglutarate. These observations are discussed with reference to the deduced amino acid sequences of the constituent subunits of the enzymes, where they are available.
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October 1994
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Research Article|
October 15 1994
Comparison of the effects of Ca2+, adenine nucleotides and pH on the kinetic properties of mitochondrial NAD+-isocitrate dehydrogenase and oxoglutarate dehydrogenase from the yeast Saccharomyces cerevisiae and rat heart
B J Nichols;
B J Nichols
*Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 lTD, U.K.
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M Rigoulet;
M Rigoulet
†Institut de Biochimie Cellulaire, CNRS, 1 Rue Camille Saint-Saëns, 33077 Bordeaux Cedex, France
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R M Denton
R M Denton
*Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 lTD, U.K.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1994 The Biochemical Society, London
1994
Biochem J (1994) 303 (2): 461–465.
Citation
B J Nichols, M Rigoulet, R M Denton; Comparison of the effects of Ca2+, adenine nucleotides and pH on the kinetic properties of mitochondrial NAD+-isocitrate dehydrogenase and oxoglutarate dehydrogenase from the yeast Saccharomyces cerevisiae and rat heart. Biochem J 15 October 1994; 303 (2): 461–465. doi: https://doi.org/10.1042/bj3030461
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