By a confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) we have studied thapsigargin-induced calcium signals in individual rat basophilic leukaemia (RBL-2H3) cells. In the presence or absence of external calcium ions, thapsigargin-induced calcium signals were transferred to the nucleus as well as to the cytoplasm of RBL-2H3 cells. The calcium signals were generally much stronger in the nucleus than in the cytoplasm. However, some of the RBL-2H3 cells had apparently reduced nuclear calcium signals. They had a basophil-like bilobed (multilobed) nucleus, although most RBL-2H3 cells had a mast-cell-like monolobed nucleus. In the cells with a bilobed nucleus, IgE-receptor-mediated calcium signals were neither transferred to the nucleus nor to the cytoplasm. The results gave a new insight into the understanding of the mechanism of the nuclear calcium signals in RBL-2H3 cells.
Research Article| November 15 1994
Thapsigargin-induced nuclear calcium signals in rat basophilic leukaemia cells
Biochem J (1994) 304 (1): 57–60.
- Views Icon Views
- Share Icon Share
Y Horikoshi, T Furuno, R Teshima, J Sawada, M Nakanishi; Thapsigargin-induced nuclear calcium signals in rat basophilic leukaemia cells. Biochem J 15 November 1994; 304 (1): 57–60. doi: https://doi.org/10.1042/bj3040057
Download citation file: