The involvement of a carrier for sinefungin (SF) uptake in Leishmania donovani promastigotes is indicated by saturation kinetics, competition studies and SF accumulation against a 270-fold concentration gradient across the cell membrane. Whether SF uptake occurs via nucleoside- or AdoMet-carrier systems was investigated by competition experiments and comparison of the uptake of various molecules in wild-type and SF-resistant cells. Results show that SF did not inhibit purine or pyrimidine uptake whereas it competitively inhibited AdoMet uptake. Furthermore, the uptake of nucleosides in SF-resistant cells is similar to that in wild-type cells, whereas uptake of SF and AdoMet is lower.
Research Article| January 01 1995
Sinefungin shares AdoMet-uptake system to enter Leishmania donovani promastigotes
M A Phelouzat;
Biochem J (1995) 305 (1): 133–137.
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M A Phelouzat, M Basselin, F Lawrence, M Robert-Gero; Sinefungin shares AdoMet-uptake system to enter Leishmania donovani promastigotes. Biochem J 1 January 1995; 305 (1): 133–137. doi: https://doi.org/10.1042/bj3050133
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