The mechanism of Ca2+ entry after ligand binding to receptors on the surface of non-excitable cells is a current focus of interest. Considerable attention has been given to Ca2+ influx induced by emptying of intracellular pools. Thapsigargin, an inhibitor of microsomal Ca(2+)-ATPase, is an important tool in inducing store-regulated Ca2+ influx. In the present paper we show that, at concentrations above 500 nM, thapsigargin also has an opposite effect: it inhibits store-regulated Ca2+ influx into Fura-2-loaded human neutrophil granulocytes. As thapsigargin has been frequently applied at concentrations up to 2 microM, its inhibitory action on plasma-membrane Ca2+ fluxes deserves consideration.
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Research Article| January 15 1995
Thapsigargin inhibits Ca2+ entry into human neutrophil granulocytes
J B Szeberényi;
Biochem J (1995) 305 (2): 525–528.
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M Geiszt, K Káldi, J B Szeberényi, E Ligeti; Thapsigargin inhibits Ca2+ entry into human neutrophil granulocytes. Biochem J 15 January 1995; 305 (2): 525–528. doi: https://doi.org/10.1042/bj3050525
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