A 50 kDa protein that inhibits platelet adhesion to collagen has been isolated from snake venom of Crotalus atrox (western diamondback rattlesnake) and has been named ‘catrocollastatin’. The cDNA cloning of catrocollastatin has been accomplished. A full-length cDNA of 2310 bp with an open reading frame between nucleotides 51 and 1880 was obtained. The deduced amino acid sequence consists of 609 amino acids. The cDNA-predicted amino acid sequence is highly similar to that of haemorrhagic metalloproteinase jararhagin from Bothrops jararaca venom, HR1B from Trimeresurus flavoviridis, Ht-e from C. atrox and trigramin from T. gramineus. Like jararhagin and HR1B, catrocollastatin is a multidomain molecule composed of an N-terminal domain, a metalloproteinase domain, a disintegrin-like domain and a cysteine-rich C-terminal domain. In the disintegrin-like domain, the frequently seen RGD (Arg-Gly-Asp) sequence is replaced by SECD (Ser-Glu-Cys-Asp). This cDNA was expressed in Spodoptera frugiperda (fall armyworm) (Sf9) insect cells using a baculovirus expression system. Like native catrocollastatin, the expressed protein is capable of selectively blocking collagen-induced platelet aggregation. This is the first full-length clone of a high-molecular-mass haemorrhagin to be expressed.
Skip Nav Destination
Article navigation
April 1995
- Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Research Article|
April 15 1995
Molecular cloning and expression of catrocollastatin, a snake-venom protein from Crotalus atrox (western diamondback rattlesnake) which inhibits platelet adhesion to collagen
Q Zhou;
Q Zhou
*Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
Search for other works by this author on:
J B Smith;
J B Smith
*Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
Search for other works by this author on:
M H Grossman
M H Grossman
†Deparment of Pediatrics, Temple University School of Medicine,St. Christopher's Hospital for Children, Philadelphia, PA 19134, U.S.A.
Search for other works by this author on:
Biochem J (1995) 307 (2): 411–417.
Citation
Q Zhou, J B Smith, M H Grossman; Molecular cloning and expression of catrocollastatin, a snake-venom protein from Crotalus atrox (western diamondback rattlesnake) which inhibits platelet adhesion to collagen. Biochem J 15 April 1995; 307 (2): 411–417. doi: https://doi.org/10.1042/bj3070411
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.