Glial hyaluronate-binding protein (GHAP) is a 60 kDa glycoprotein with an amino acid sequence identical to that of the hyaluronate-binding region of versican, a large fibroblast aggregating proteoglycan found in the brain. Both GHAP and versican were identified by immunoblot in bovine brain extracts prepared only minutes after death. Human recombinant collagenase, stromelysin, mouse gelatinase and gelatinases isolated from human brain by affinity chromatography digest versican and give rise to a polypeptide with electrophoretic mobility identical to GHAP. Immunoblot analysis, peptide mapping and C-terminal amino acid sequencing indicate that the polypeptide generated by digestion with human brain gelatinases is identical to GHAP. We suggest that GHAP is a naturally occurring versican degradation product.
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December 1995
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Research Article|
December 01 1995
Glial hyaluronate-binding protein: a product of metalloproteinase digestion of versican? Available to Purchase
G Perides;
G Perides
†Harvard Medical School, Department of Pathology and Department of Veterans Affairs, West Roxbury Medical Center, 1400 VFW Parkway, Boston, MA 02132, U.S.A.
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R A Asher;
R A Asher
†Harvard Medical School, Department of Pathology and Department of Veterans Affairs, West Roxbury Medical Center, 1400 VFW Parkway, Boston, MA 02132, U.S.A.
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M W Lark;
M W Lark
‡Merck Research Laboratories, P.O. Box 2000, Rahway, NJ 07065, U.S.A.
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W S Lane;
W S Lane
§Biological Laboratories, Microchemistry Facility, Harvard University, Cambridge, MA 02138, U.S.A.
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R A Robinson;
R A Robinson
§Biological Laboratories, Microchemistry Facility, Harvard University, Cambridge, MA 02138, U.S.A.
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A Bignami
A Bignami
†Harvard Medical School, Department of Pathology and Department of Veterans Affairs, West Roxbury Medical Center, 1400 VFW Parkway, Boston, MA 02132, U.S.A.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1995 The Biochemical Society, London
1995
Biochem J (1995) 312 (2): 377–384.
Citation
G Perides, R A Asher, M W Lark, W S Lane, R A Robinson, A Bignami; Glial hyaluronate-binding protein: a product of metalloproteinase digestion of versican?. Biochem J 1 December 1995; 312 (2): 377–384. doi: https://doi.org/10.1042/bj3120377
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