The potential regulatory role of delta protein kinase C (delta PKC) in murine erythroleukaemia cell differentiation was studied by using antisense oligodeoxynucleotides targeting the translation initiation region of mouse delta PKC mRNA. Cell treatment with antisense oligonucleotides, at a concentration of 20 microM, followed by hexamethylenebisacetamide induction, produced a specific 2-fold increase in the differentiation rate of both slowly and rapidly differentiating murine erythroleukaemia cell clones. Cell permeabilization by a cationic lipid resulted in a decrease of one order of magnitude in the amounts of antisense oligonucleotides necessary to elicit the maximal response, and accelerated the kinetics of the stimulatory effect. These changes in murine erythroleukaemia cell differentiation rates, observed in both cell clones, were associated with 60% and 50% decreases, respectively, in delta PKC immunoreactive protein in slowly and rapidly differentiating cells. The present results indicate strongly that basal levels of delta PKC in murine erythroleukaemia cells are essential in regulating the initial differentiation rate of these cells in response to chemical induction, and provide further evidence that this PKC isoform plays a fundamental role in maintaining the undifferentiated phenotype of murine erythroleukaemia cells.
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December 1995
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December 01 1995
Antisense oligodeoxynucleotide inhibition of δ protein kinase C expression accelerates induced differentiation of murine erythroleukaemia cells Available to Purchase
A Pessino;
A Pessino
1Institute of Biological Chemistry, University of Genova, Viale Benedetto XV no. 1, 16132 Genova, Italy
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M Passalacqua;
M Passalacqua
1Institute of Biological Chemistry, University of Genova, Viale Benedetto XV no. 1, 16132 Genova, Italy
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B Sparatore;
B Sparatore
1Institute of Biological Chemistry, University of Genova, Viale Benedetto XV no. 1, 16132 Genova, Italy
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M Patrone;
M Patrone
1Institute of Biological Chemistry, University of Genova, Viale Benedetto XV no. 1, 16132 Genova, Italy
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E Melloni;
E Melloni
1Institute of Biological Chemistry, University of Genova, Viale Benedetto XV no. 1, 16132 Genova, Italy
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S Pontremoli
S Pontremoli
1Institute of Biological Chemistry, University of Genova, Viale Benedetto XV no. 1, 16132 Genova, Italy
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1995 The Biochemical Society, London
1995
Biochem J (1995) 312 (2): 549–554.
Citation
A Pessino, M Passalacqua, B Sparatore, M Patrone, E Melloni, S Pontremoli; Antisense oligodeoxynucleotide inhibition of δ protein kinase C expression accelerates induced differentiation of murine erythroleukaemia cells. Biochem J 1 December 1995; 312 (2): 549–554. doi: https://doi.org/10.1042/bj3120549
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