The formation of S-nitrosoglutathione (GSNO) from amyl nitrite and n-butyl nitrite was studied in rat liver microsomes, employingN-ethylmaleimide (MalNEt) as an activator and indomethacin as an inhibitor of microsomal glutathione S-transferase (GST). Rates were compared with GST activity measured with 1-chloro-2,4-dinitrobenzene(CDNB) as a substrate. MalNEt stimulated GST activity and the formation of GSNO from amyl nitrite and n-butyl nitrite about 10-fold. Increasing concentrations of indomethacin inhibited both reactions in parallel. N-Acetyl-L-cysteine but not L-cysteine could substitute for GSH. It is concluded that rat liver microsomal GST catalyses the formation of GSNO from amyl nitrite and n-butyl nitrite. The activity of the MalNEt-stimulated microsomal GST is calculated to be about 17 units/mg of enzyme with the alkyl nitrites and about 16 units/mg of enzyme with CDNB as a substrate, assuming that 3% of microsomal protein is GST. These rates are comparable with those obtained for cytosolic GSTs. Thus microsomal GST may play a significant role in the metabolism of alkyl nitrites in biological membranes.
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January 1996
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Research Article|
January 15 1996
Microsomal formation of S-nitrosoglutathione from organic nitrites: possible role of membrane-bound glutathione transferase Available to Purchase
Yanbin JI;
Yanbin JI
1Institut für Physiologische Chemie I, Heinrich-Heine Universität Düsseldorf, Postfach 101007, D-40001 Düsseldorf, Federal Republic of Germany
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Theodorus P. M. AKERBOOM;
Theodorus P. M. AKERBOOM
1Institut für Physiologische Chemie I, Heinrich-Heine Universität Düsseldorf, Postfach 101007, D-40001 Düsseldorf, Federal Republic of Germany
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Helmut SIES
Helmut SIES
*
*To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
May 31 1995
Revision Received:
September 06 1995
Accepted:
September 13 1995
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 313 (2): 377–380.
Article history
Received:
May 31 1995
Revision Received:
September 06 1995
Accepted:
September 13 1995
Citation
Yanbin JI, Theodorus P. M. AKERBOOM, Helmut SIES; Microsomal formation of S-nitrosoglutathione from organic nitrites: possible role of membrane-bound glutathione transferase. Biochem J 15 January 1996; 313 (2): 377–380. doi: https://doi.org/10.1042/bj3130377
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