Neutral oligomannosides possessing one GlcNAc (OS-Gn1) and two GlcNAc (Os-Gn2) at the reducing end have been reported to be released during the N-glycosylation process in various biological models. To investigate which enzyme is responsible for OS-Gn1 formation, we used the Madin-Darby bovine kidney (MDBK) cell line which exhibits neither lysosomal chitobiase nor endoglucosaminidase activities. However, these cells produced OS-Gn1 and we showed that a neutral chitobiase is responsible for the transformation of OS-Gn2 into OS-Gn1. Using streptolysin O-permeabilized MDBK cells, we demonstrated that this neutral chitobiase activity is located in the cytosolic compartment and is active on oligomannoside species released during the N-glycosylation process.
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January 1996
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Research Article|
January 15 1996
Occurrence of a cytosolic neutral chitobiase activity involved in oligomannoside degradation: a study with Madin-Darby bovine kidney (MDBK) cells Available to Purchase
René CACAN;
René CACAN
1Laboratoire de Chimie Biologique, UMR 111 du C.N.R.S., Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cédex, France
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Catherine DENGREMONT;
Catherine DENGREMONT
1Laboratoire de Chimie Biologique, UMR 111 du C.N.R.S., Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cédex, France
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Odette LABIAU;
Odette LABIAU
1Laboratoire de Chimie Biologique, UMR 111 du C.N.R.S., Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cédex, France
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Daniel KMIÉCIK;
Daniel KMIÉCIK
1Laboratoire de Chimie Biologique, UMR 111 du C.N.R.S., Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cédex, France
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Anne-Marie MIR;
Anne-Marie MIR
1Laboratoire de Chimie Biologique, UMR 111 du C.N.R.S., Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cédex, France
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André VERBERT
André VERBERT
*
*To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
June 15 1995
Revision Received:
September 11 1995
Accepted:
September 12 1995
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 313 (2): 597–602.
Article history
Received:
June 15 1995
Revision Received:
September 11 1995
Accepted:
September 12 1995
Citation
René CACAN, Catherine DENGREMONT, Odette LABIAU, Daniel KMIÉCIK, Anne-Marie MIR, André VERBERT; Occurrence of a cytosolic neutral chitobiase activity involved in oligomannoside degradation: a study with Madin-Darby bovine kidney (MDBK) cells. Biochem J 15 January 1996; 313 (2): 597–602. doi: https://doi.org/10.1042/bj3130597
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