Na+-dependent uridine uptake is stimulated in isolated rat liver parenchymal cells by glucagon. This effect is transient, reaches maximum levels of stimulation 10 min after hormone addition, and is dose-dependent. Glucagon action can be mimicked by agents that are able to hyperpolarize the plasma membrane (e.g. monensin) and by dibutyryl cyclic AMP. The effects triggered by glucagon, monensin and dibutyryl cyclic AMP are not additive, suggesting a common mechanism of action. 8-(4-Chlorophenylthio)adenosine 3´:5´-cyclic monophosphate (PCT), a cyclic AMP analogue but also a nucleoside analogue, markedly stimulates Na+-dependent uridine uptake in an additive manner to that triggered by monensin, similarly to the effect described for nitrobenzylthioinosine. Considering the roles reported for nucleosides in liver metabolism, the use of PCT as a cyclic AMP analogue should be precluded. Insulin is also able to up-regulate Na+-dependent uridine uptake by a mechanism which involves a stable induction of this transport activity at the plasma-membrane level. This is consistent with a mechanism involving synthesis and insertion of more carriers into the plasma membrane. It is concluded that the recently characterized hepatic concentrative nucleoside transporter is under short-term hormonal regulation by glucagon, through mechanisms which involve membrane hyperpolarization, and under long-term control by insulin. This is the first report showing hormonal modulation of the hepatic concentrative nucleoside transporter.
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Research Article|
February 01 1996
Hormonal regulation of concentrative nucleoside transport in liver parenchymal cells
Mireia GOMEZ-ANGELATS;
Mireia GOMEZ-ANGELATS
1Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain
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Belén del SANTO;
Belén del SANTO
1Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain
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Joan MERCADER;
Joan MERCADER
1Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain
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Andreu FERRER-MARTINEZ;
Andreu FERRER-MARTINEZ
1Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain
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Antonio FELIPE;
Antonio FELIPE
1Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain
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Javier CASADO;
Javier CASADO
1Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain
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Marçal PASTOR-ANGLADA
Marçal PASTOR-ANGLADA
*
1Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain
*To whom correspondence and reprint requests should be addressed.
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Publisher: Portland Press Ltd
Received:
August 01 1995
Revision Received:
September 25 1995
Accepted:
October 05 1995
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 313 (3): 915–920.
Article history
Received:
August 01 1995
Revision Received:
September 25 1995
Accepted:
October 05 1995
Citation
Mireia GOMEZ-ANGELATS, Belén del SANTO, Joan MERCADER, Andreu FERRER-MARTINEZ, Antonio FELIPE, Javier CASADO, Marçal PASTOR-ANGLADA; Hormonal regulation of concentrative nucleoside transport in liver parenchymal cells. Biochem J 1 February 1996; 313 (3): 915–920. doi: https://doi.org/10.1042/bj3130915
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