The glycosylation of tissue plasminogen activator (t-PA) obtained from the Bowes melanoma cell line was re-examined using methods of serial lectin affinity chromatography coupled with Bio-Gel P-4 gel filtration chromatography and exoglycosidase sequencing. This study clarified an earlier discrepancy in the literature and confirmed that the major complex N-linked glycans on Bowes t-PA that carry sialic acid as their sole charged group are bi-antennary, core fucosylated, with terminal N-acetylgalactosamine residues. We also report the characterization of a series of related and previously unidentified sialylated glycans. Further we show that Bowes t-PA expresses glucuronic acid/sulphate containing N-linked glycans and is recognized by anti-carbohydrate L2/HNK-1 monoclonal antibodies. The presence on Bowes t-PA of glycans associated primarily with the nervous system is consistent with its expression in a cell line of neuroectodermal origin.
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Research Article|
June 01 1996
The glycosylation of Bowes melanoma tissue plasminogen activator: lectin mapping, reaction with anti-L2/HNK-1 antibodies and the presence of sulphated/glucuronic acid containing glycans
A. J. JAQUES;
A. J. JAQUES
1Glycobiology Institute, Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, U.K.
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G. OPDENAKKER;
G. OPDENAKKER
1Glycobiology Institute, Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, U.K.
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T. W. RADEMACHER;
T. W. RADEMACHER
1Glycobiology Institute, Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, U.K.
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R. A. DWEK;
R. A. DWEK
1Glycobiology Institute, Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, U.K.
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S. E. ZAMZE
S. E. ZAMZE
*
1Glycobiology Institute, Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, U.K.
*To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
September 04 1995
Revision Received:
January 11 1996
Accepted:
January 24 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 316 (2): 427–437.
Article history
Received:
September 04 1995
Revision Received:
January 11 1996
Accepted:
January 24 1996
Citation
A. J. JAQUES, G. OPDENAKKER, T. W. RADEMACHER, R. A. DWEK, S. E. ZAMZE; The glycosylation of Bowes melanoma tissue plasminogen activator: lectin mapping, reaction with anti-L2/HNK-1 antibodies and the presence of sulphated/glucuronic acid containing glycans. Biochem J 1 June 1996; 316 (2): 427–437. doi: https://doi.org/10.1042/bj3160427
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