Insulin-dependent diabetes mellitus (IDDM) is an autoimmune disease in which cytokines are thought to play an important role in β-cell destruction and immune regulation. A major target of β-cell autoimmunity in IDDM is the enzyme glutamate decarboxylase (GAD). We hypothesized that cytokines in the insulitis lesion modulate the synthesis of GAD. This may, in turn, modify the rate of β-cell destruction. Accordingly we cultured rat islets in the presence and absence of cytokines, and measured synthesis of both isoforms of GAD, GAD65 and GAD67, by [35S]methionine incorporation and immunoprecipitation with a rabbit antiserum that recognizes both GAD65 and GAD67. Incubation of islets with interleukin (IL)-1β (1 ng/ml, 24 h), tumour necrosis factor α (TNF-α; 200 units/ml, 24 h) or interferon γ (IFN-γ; 500 units/ml, 72 h) significantly decreased the synthesis of both GAD65 and GAD67, but reduced neither total protein synthesis nor insulin accumulation in the medium or content. Incubation of islets for 24 h in IFN-α (1000 units/ml), TNF-β (50 ng/ml), IL 2 (1000 units/ml), IL-4 (100 ng/ml), IL-6 (10 ng/ml), IL-10 (20 ng/ml), IL-12 (10 ng/ml) or transforming growth factor β2 (TGF-β2; 5 ng/ml) did not significantly alter GAD65 or GAD67 synthesis. Inhibition of GAD65 and GAD67 protein synthesis by IL-1β, TNF-α or IFN-γ was reversed by co-incubation with the nitric oxide synthase inhibitor, NG-monomethyl arginine (NMMA). Expression of both GAD65 and GAD67 mRNA, measured by RNase protection assay, was also decreased by IL-1β and completely restored to baseline levels by NMMA. Thus the synthesis of both isoforms of islet GAD is selectively decreased in the presence of IL-1β, TNF-α or IFN-γ by a NO-mediated mechanism, probably at the level of cytokine gene transcription. As GAD autoimmunity has been previously shown to have a pathogenic role in an animal model of IDDM, its inhibition by cytokines might limit the immune response, thereby regulating the rate of β-cell destruction in IDDM.
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August 1996
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Research Article|
August 01 1996
Cytokine regulation of glutamate decarboxylase biosynthesis in isolated rat islets of Langerhans
Robert S. SCHMIDLI
;
Robert S. SCHMIDLI
*Burnet Clinical Research Unit, Walter and Eliza Hall Institute of Medical Research, c/o Post Office, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia
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Beverly E. FAULKNER-JONES
;
Beverly E. FAULKNER-JONES
†Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Victoria 3052, Australia
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Leonard C. HARRISON
;
Leonard C. HARRISON
*Burnet Clinical Research Unit, Walter and Eliza Hall Institute of Medical Research, c/o Post Office, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia
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Roger F. L. JAMES
;
Roger F. L. JAMES
‡Department of Surgery, University of Leicester, Leicester Royal Infirmary, P.O. Box 65, Leicester LE2 7LX, U.K.
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Henry J. DeAIZPURUA
Henry J. DeAIZPURUA
§
*Burnet Clinical Research Unit, Walter and Eliza Hall Institute of Medical Research, c/o Post Office, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia
§To whom correspondence should be addressed.
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Biochem J (1996) 317 (3): 713–719.
Article history
Received:
January 24 1996
Revision Received:
March 15 1996
Accepted:
March 21 1996
Citation
Robert S. SCHMIDLI, Beverly E. FAULKNER-JONES, Leonard C. HARRISON, Roger F. L. JAMES, Henry J. DeAIZPURUA; Cytokine regulation of glutamate decarboxylase biosynthesis in isolated rat islets of Langerhans. Biochem J 1 August 1996; 317 (3): 713–719. doi: https://doi.org/10.1042/bj3170713
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